The glidine used in the protein experiments represented an approximately pure vegetable protein; the gluten was also a vegetable protein. To study the effect of an animal protein, plasmon, a food material derived from milk, was given to the subject in two experiments. Bo experiments were made with the Middletown respiration calorimet in 2-hour periods.

H. R. D., May 4, 1906

In addition to 100 grams of plasmon, the subject took 70 grams of plasmon milk biscuit and 206 grams of skim milk. The total nitrogen intake was 15.07 grams, of which 11.92 grams came from the plasmon, 2.10 grams from the plasmon milk biscuit, and 1.05 grams from the skim milk. With this diet 36 per cent of the fuel value of the intake was derived from carbohydrates and but 54 per cent from protein. The basal value employed was the average of determinations made between February 6 and April 20 of the same year. As may be seen from table 228, an increment in carbon-dioxide excretion was found in all four periods. The measurement of oxygen consumption was not obtained for the first period, but subsequently a pronounced increment was observed. The heat production also increased in the four periods and the nitrogen excretion was very considerably increased. The body-temperature approximated 36.7° C.; the pulse rate averaged 65 and the respiration rate 19. The ingestion of the 15 grams of nitrogen in this diet thus resulted in a pronounced increase in the metabolism, which continued for at least 8 hours.

Table 228. - H. R. D., May 4, 1906. Sitting. (2-Hour Periods). Plasmon, Plasmon Milk Biscuit, And Skim Milk

Amounts, 100 grams plasmon, 70 grams plasmon milk biscuit, 206 grams skim milk; nitrogen, 15.07 grams; total energy, 800 cals. Fuel value: Total, 758 cals.; from protein, 54 p. ct.; from fat, 10 p. ct.; from carbohydrates, 36 p. ct. Basal values (February 6 to April 20, 1906): CO2. 47 grams; O2. 42 grams; heat, 146 cals. Nitrogen in urine, 0.77 gram per 2 hours (May 4, 1906).

1Subject ate food in 36 minutes.

2Increment of oxygen 2 to 8 hours after food.

H. C. K., May 15, 1906

Plasmon graham biscuit was substituted for the milk biscuit used in the previous experiment, the amounts being 100 grams plasmon, 47 grams plasmon graham biscuit, and 439 grams skim milk. The total nitrogen intake was practically the same as in the first experiment, i. e., 15.25 grams, with 11.92 grams from the plasmon, 1.07 grams from the biscuit, and 2.26 grams from the skim milk. The experiment was lengthened to 12 hours in order to obtain the total effect of the food. The results of the experiment are given in table 229, which shows that the increment in carbon-dioxide production persists throughout the entire experiment. The values obtained for oxygen consumption were variable, as is indicated by the great irregularity in the respiratory quotients. The possibility of a compensation can hardly be considered here and the variations are undoubtedly due to some technical difficulty. The measurements of the heat production show positive increments during the first three periods, with values lower than basal in the fourth period, an increment in the fifth period, and a value lower than basal in the sixth period. The nitrogen values are characteristic, with a maximum in the third period. The body-temperature averaged 36.7° C; the pulse rate averaged 51 and the respiration rate 18. Unfortunately the results obtained in this experiment do not permit definite conclusions regarding either the amount or the duration of the effect upon the metabolism due to the ingestion of this amount of nitrogen. The general effect of 100 grams or more of plasmon, together with 200 c.c. or over of skim milk, is to increase the heat output considerably above the basal metabolism for at least 10 hours.

Table 229. - H. C. K., May 15, 1906. Sitting. (2-Hour Periods). Plasmon, Plasmon Graham Biscuit, Skim Milk

Amounts, 100 grams plasmon, 47 grams plasmon graham biscuit, 439 grams skim milk; nitrogen, 15.25 grams; total energy, 862 cals. Fuel value: Total, 728 cals.; from protein, 56 p. ct.; from fat, 8 p. ct.; from carbohydrates, 36 p. ct. Basal values (May 3, 1906): CO2, 51 grams; O2, 47 grams; heat, 164 cals. Nitrogen in urine, 0.97 gram per 2 hours (May 15, 1906).1

1Sample included amount for about 1 hour from the time subject began to eat food. 2Subject ate food in 34 minutes.