Following the construction of the respiration calorimeters1 in the Nutrition Laboratory, a series of five experiments subsequent to the ingestion of beefsteak was made with the chair calorimeter, beginning on December 4, 1908. The first two were 8-hour experiments, but the last three experiments continued for only 5 or 6 hours. The observations were made in 1-hour periods in all cases. The statistical data not included in the tables or discussion of these experiments are given in the following paragraphs; no additional data were available for L. E. E.:

J. R., 9h45m A. M. To 5h45m P. M., December 4, 1908. 66.2 Kilograms

Drank water at 10 a. m. (198 grams); urinated 7, 9h57m, llh58m a. m., 2, 3h59m, and 5h55m p. m. Head ached after 2h30m p. m. Pulse rate, 62; these records sometimes difficult to obtain and at times pulse rate very low. No records could be obtained last half hour. Respiration rate, 17; but one record obtained after 4h45m p. m. Nitrogen in urine per hour 7 a. m. to 9h57m a. m., 0.48 gram.

F. M. M., 9h01m A. M. To 5h01m P. M., December 10, 1918. 59.8 Kilograms

In seventh period showed tendency to fall asleep; instructed to ring signal bell every five minutes. Urinated 7h05m, 9h01m, llh01m a. m., lh01m, 3h01m, and 5h01m p. m. Drank water at 9h01m a. m., llh01m a. m., 2h01m p. m., and 4h01m p. m. (total amount, 998 grams). Pulse rate, 52. Nitrogen in urine per hour 7h05m a. m. to 9h01m a. m., 0.48 gram.

F. M. M., 8h55m A. M. To 2h55m P. M., December 23, 1908. 59.8 Kilograms

Did not eat all beefsteak provided, owing to pain in stomach; feared that a larger amount might increase pain and prevent experiment. Urinated and defecated at 7h30m a. m., also urinated at 11 a. m., 1 p.m., and 3 p.m.; drank water at 9 a. m., 11 a. m., and 1 p. m. (681 grams in all). Body-temperature: 36.75°, 36.95°, 36.79°, 36.69°, 36.66°, 36.72°, 36.85° C. Pulse rate, 56.

1Benedict and Carpenter, Carnegie Inst. Wash. Pub. No. 123, 1910.

F. M. M., 9h26m A. M. To 3h06m P. M., January 20, 1910. 61.5 Kilograms

Third period lengthened owing to unaccountable variations in the temperature conditions at end of hour. Nitrogen in urine per hour 6h45ma.m. to 10h30m a. m., 0.85 gram.

Discussion Of Experiments

J. R., December 4, 1908

The basal value for this experiment was determined on December 3. After the ingestion of 418 grams of beefsteak on December 4, having a nitrogen content of 15.30 grams, an increment in the carbon-dioxide production was noted in all of the eight periods, although the values had nearly reached the basal level in the last period (see table 202). Noticeable increments in the oxygen consumption were also found throughout the experiment; even in the eighth hour there was a consumption of 4 grams more than the basal requirement. The heat production was likewise increased in all of the periods. The maximum increments for carbon-dioxide production and oxygen consumption were noted in the third hour of the experiment, while that for heat production was found in the first period. The total increment, both in carbon-dioxide production and in oxygen consumption, was 31.5 grams; in heat production it was 104 calories. As the basal level had not been reached at the end of the experiment, the stimulus of the beefsteak was apparently still effective. The large excretion of nitrogen in the urine, with a total increment of 7.80 grams for the 8 periods, indicates a considerable katabolism of protein during the experiment.

Table 202. - J. R., December 4, 1908. Sitting. (1-Hour Periods). Beefsteak

Amount, 418 grams; nitrogen, 15.30 grams; total energy, 737 cals.

Fuel value: Total, 603 cals.; from protein, 65 p. ct.; from fat, 35 p. ct. Basal value* (December 3, 1908): CO2, 26.5 grams; O2, 23.5 grams.; heat,1 74 cals. Nitrogen in urine, 0.38 gram per hour (December 4, 1908).

1Heat eliminated corrected for change in body-weight, but not for change in body-temperature.