Records were kept of the milk production during the first thirty days of the first lactation period. The average production per day per each individual of the corn-fed lot was 24.03 pounds; for the wheat-fed animals 8.04, and for the oat-fed animals 19.38 pounds. Those fed the mixture of the three plants produced an average of 19.82 pounds of milk per cow per day during the first thirty days. In the second lactation period the average figures for milk production were 28.0, 16.1, 30.1 and 21.3 pounds, respectively, per day during the first thirty days.

Through autopsy and analysis of the tissues of the young, and analysis of the feeds and excreta of the animals of the several groups, an elaborate attempt was made to solve the problem of the cause of the marked differentiation of the animals fed these restricted diets. Interesting data were secured which showed marked differences in the character of the fat in the milk of the cows of the different lots. The observation was made that the urine of the wheat-fed animals was invariably distinctly acid in reaction, whereas that of the other lots was alkaline or neutral. It was not possible by any means known to biological chemistry to work out a reason as to the cause of the pronounced differences in the physiological well-being of the different lots of cows.