F. M. M., 10h24m A. M. To 4h24m P. M., April 8, 1909. 59.4 Kilograms. 3 Basal Periods

Urinated and defecated at 9h05ma. m., urinated at lh32m p. m. and immediately after experiment. At end of third basal period, asleep for about 20 minutes, waking up just before end of period, then unusually active. Considerable telephoning at beginning of periods in connection with weighings. Restless during last food period. Basal periods: pulse-rate, 52; respiration rate, 15. After food: pulse rate, 57; respiration rate, 17.

F. M. M., 9h38m A. M. To 3h38m P. M., February 8, 1910. 61.8 Kilograms. 2 Basal Periods

Urinated 7 a. m., llh40m a. m., 3h50m p. m. Drank water 9h45m, 10h58m, llh55m a. m., 12h55m p. m. (230 grams in all). At end of first basal period and beginning of second, restless. During last food period, asleep part of time but quite restless whenever awake; was required to press push button to ring bell outside, thus indicating that he was awake. Basal periods: pulse rate, 61; respiration rate, 13. Food periods: pulse rate, 59; respiration rate, 14.

Dr. H., 9h24m A. M. To 2h24m P.m., February 14, 1910. 66.6 Kilograms. 2 Basal Periods

Urinated 8, 9h28m, llh32m a. m., lh30m, 2h30m p. m. Drank water llh36m a. m. (135 grams). Basal periods: pulse rate, 58; respiration rate, 13. Food periods: pulse rate, 61; respiration rate, 14.

Dr. H., 9h31m A. M. To 3h31m P. M., February 17, 1910. 66.0 Kilograms. 2 Basal Periods

Urinated 8, 9h40m, llh36m a. m., 2h50m, 3h31m p. m. Drank water at llh45m a. m. (112 grams). Basal periods: pulse rate, 59; respiration rate, 12. Food periods: pulse rate, 62; respiration rate, 13.

H. B. W., 9h44m A. M. To 5h44m P. M., April 9, 1907. 62.6 Kilograms

Defecated before coming to laboratory; urinated at 8h20m a. m. Very quiet nearly all of experimental period, most movement being in second and third periods. Head ached last period, probably due to reading steadily. Body-temperature: 36.80°, 36.79°, 36.85°, 36.93°, 36.97° C. Pulse rate, 59; respiration rate, 18.

A. H. M., 9h23m A. M. To 5h23m P. M., April 10, 1907. 66.6 Kilograms

Urinated 7h30m, llh32m a. m., 3h30m p. m.; attempted to urinate at lh30m p. m. Somewhat restless throughout experiment but did not rise from chair; reading much of time; seldom motionless for more than half minute at a time; difficult to get records of respiration and pulse rates; in last period more quiet; slight headache in afternoon. Body-temperature: 36.76°, 36.70°, 36.84°, 36.80°, 36.78° C. Pulse rate, 63; respiration rate, 19.

A. L. L., 8b30m A. M. To 4h30m P. M., May 27, 1907. 74.7 Kilograms

Urinated 7h05m a m.; very quiet during experiment; fell asleep in second period and had to be awakened; also slept for short time in last period. Body-temperature: 36.62°, 36.28°, 36.22°, 36.12°, 36.20° C. Pulse rate, 61; respiration rate, 18.

Discussion Of Calorimeter Experiments. Sucrose

Four experiments were made with sucrose, one each with A. H. M. and A. W. W. with the respiration calorimeter in Middletown, and two with F. M. M. with the chair calorimeter in Boston.

A. H. M., April 1, 1907

This experiment was the first in this research in which a pure carbohydrate was ingested. The amount taken was 191 grams, with a total energy value of 756 calories. An inspection of table 101 shows a considerable increase in the carbon-dioxide production after food which persisted during the first three periods but does not appear in the last period. The oxygen consumption shows a marked increase in the first period, with a return to the basal value immediately thereafter. High respiratory quotients, which usually follow the ingestion of sugar, were found in the first two periods with a quotient approximating basal in the last period. The striking abnormality in the values for this experiment is the fact that the oxygen increment appears only in the first period, while the increase in the heat production continues throughout all four periods. An explanation of this on any other ground than that of unrecognized faulty technique is at present very difficult.

As there were certain discrepancies in the measurements of the rectal temperature which led us to consider the records doubtful, it seemed desirable to compare the direct measurements of the heat output with the values calculated from the gaseous metabolism. For this particular experiment, therefore, the values obtained by indirect calorimetry are also recorded, although it should again be emphasized that the values for the indirect heat are not given for the specific purpose of noting the increment above the basal value but simply to obtain the general trend of the metabolism from period to period for comparison with the direct measurements of the heat production. Aside from the first period, in which the computed heat is 16 calories higher than the determined, all the values found by direct calorimetry are higher than those computed. The average for the entire experiment shows a discrepancy between the values obtained by the two methods of approximately 6 per cent. While this discrepancy appears very large in the light of the recent exact work of Du Bois, it should be remembered that this particular calorimeter had a very large volume and was primarily designed for 24-hour experiments. The lack of agreement between the direct and indirect calorimetry in these short periods is, therefore, not so incongruous as at first sight appears.