L. E. E., May 11, 1910

The same amount of glidine was taken as in the first experiment with this subject; the basal value was determined immediately before the observations with glidine. The results are given in table 220. Noticeable increments in the carbon-dioxide production and oxygen consumption were obtained in the three 1-hour periods, but relatively insignificant increments were found in the heat production. The absence of body-temperature measurements, with the consequent impossibility of correcting for changes in body-temperature, may have accounted for this discrepancy. The nitrogen excretion per hour was less than in any of the periods of the former experiment. The increment in both the carbon-dioxide production and oxygen consumption is too great, however, not to be taken as a positive effect of the ingestion of the glidine.

Table 220. - L. E. E., May 11, 1910. Sitting. (1-Hour Periods). Glidine1

Amount, 45 grams; nitrogen, 6.24 grams; total energy, 223 cals.

Fuel value: Total, 168 cals.; from protein, 95 p. ct.; from fat, 2 p. ct.; from carbohydrates, 3 p. ct. Nitrogen in urine, 0.40 gram per hour. Basal values (May 11,1910): CO2, 24.5 grams; O2, 21.5 grams; heat,2 80 cals.; respiratory quotient, 0.83. Nitrogen in urine, 0.10 gram per hour.

1Subject took glidine in 200 grams of water.

2Heat eliminated corrected for change in body-weight, but not for change in body-temperature.

J. J. C, May 9, 1910

A third experiment was made in which 45 grams of glidine were given, but with another subject. The results of this experiment are found in table 221, which shows a considerable increase in all the factors of the metabolism. Even at the end of the 4 hours there is no indication that the total increase due to the glidine had been obtained. The respiratory quotients are not far from those which would be expected during the combustion of protein, although it can be computed from the values obtained for the nitrogen excretion, which average 0.58 gram per hour, that the total calories from protein can be only about one-third of the total calories found, the remainder of the metabolism being derived from fat and carbohydrates.

J. R., May 5, 1910

A larger amount of glidine was taken by this subject than in the three experiments previously discussed, the exact amount being 70 grams, with a nitrogen content of 9.70 grams. The measurements of the metabolism are given in table 222. The oxygen consumption during the second period could not be obtained, but noticeable increments were found in the other periods and also for carbon-dioxide production and heat production in all the periods. It is evident that the effect of the ingestion of this amount of glidine was considerable. As the basal values were determined on the same day as the metabolism after glidine, there can be no uncertainty as to the validity of the increments.

Table 221. - J.J. C, May 9, 1910. Sitting. (1-Hour Periods). Glidine1

Amount, 45 grams; nitrogen, 6.24 grams; total energy, 223 cals.

Fuel value: Total, 168 cals.; from protein, 95 p. ct.; from fat, 2 p. ct.; from carbohydrates, 3 p. ct. Basal values: CO2, 24.5 grams (May 9, 1910); O2, 21 grams (March 4 to May 31, 1910); heat (computed), 72 cals. (May 9, 1910). Nitrogen in urine, 0.26 gram per hour (May 9, 1910).

1Subject took glidine in 164 grams of water.

Table 222. - R., May 5, 1910. Sitting. (1-Hour Periods). Glidine1

Amount, 70 grams; nitrogen, 9.70 grams; total energy, 347 cals.

Fuel value: Total, 262 cals.;.from protein, 95 p. ct.; from fat, 2 p. ct.; from carbohydrates, 3 p. ct. Nitrogen in urine, 0.83 gram per hour. Basal values (May 5, 1910): CO2, 27 grams; O2, 23 grams; heat,1 73 cals.; respiratory quotient 0.86. Nitrogen in urine, 0.44 gram per hour.

1Taken in 200 grams of water.

2Heat eliminated corrected for change in body-weight, but not for change in body-temperature.

3Increment of oxygen for a total of 3 hours.

J. R., May 10, 1910

The same amount of glidine was taken in this second experiment with J. R. as in that of May 5, and the results are therefore comparable. Increments in the carbon-dioxide excretion, oxygen consumption, and heat production were also found in this experiment, with no indication of a cessation in the stimulus at the end of the experiment. (See table 223.) The 70 grams of glidine therefore had a pronounced effect, which continued 4 hours, if not longer. The nitrogen excretion was strikingly lower in this experiment, but in any event the total energy from the protein katabolized can be but a relatively small part of the total heat production, probably about one-third.

Table 223. - J. R., May 10, 1910. Sitting. (1-Hour Periods). Glidine1

Amounts, 70 grams glidine, 20 grams lemon juice; nitrogen, 9.70 grams; total energy, 352 cals. Fuel value: Total, 267 cals.; from protein, 93 p. ct.; from fat, 2 p. ct.; from carbohydrates, 5 p. ct. Nitrogen in urine, 0.72 gram per hour (in first three periods). Basal values: C02, 27.5 grams (May 10, 1910); 02, 22.5 grams (March 21 to May 13, 1910); heat, 72 cals. (May 10, 1910). Nitrogen in urine, 0.44 gram per hour (May 10, 1910).

1Taken with lemon juice and 400 grams of water.

Conclusions As To Effect Of Ingesting Glidine

An examination of the results obtained in this series of five experiments with glidine shows that it produced a marked effect upon the metabolism even when such small amounts were taken as 45 grams, with a nitrogen content of approximately 6.25 grams. The two experiments with J. R., in which 70 grams were taken, gave a much larger increment and a more prolonged effect than the smaller amount. A comparison of these results with glidine and those obtained with other predominatingly protein diets will be made subsequently.