In studying heat production in the body under varying conditions, one of the important aids in drawing conclusions as to the character of the body material burned up is the respiratory quotient. This is the relationship, or ratio, of the oxygen absorbed to the oxygen of the carbon dioxide eliminated, viz., CO2/O2 Carbohydrates (C6H12 06, C12H22O11) all contain hydrogen and oxgeen in the proportion * fa. water, H2O, and in their oxidation they need of oxygen only such quantity as will suffice to oxidize the carbon (C) of the sugar to carbon dioxide (CO2). Carbohydrates, starch and sugars, have a respiratory quotient of 1.00. Fat, on the other hand, has a respiratory quotient of 0.7, and proteid, 0.8. Hence, it is easy to see that the respiratory quotient will approach nearer to unity as the quantity of carbohydrate burned in the body is increased. Similarly, the respiratory quotient will grow smaller the larger the amount of fat burned up. Practically, we never find a respiratory quotient of 1.0 or 0.7, because there is always some oxidation of proteid in the body. If, by way of illustration, we assume that the energy of the body under given conditions comes from proteid to the extent of 15 per cent, while the remaining 85 per cent is derived from the oxidation of carbohydrate, the respiratory quotient will be 0.971. If, however, the 85 per cent of energy comes from fat, the respiratory quotient will change to 0.722. In the resting body, as in the early morning hours, after a night's sleep and before food is taken, the respiratory quotient is generally in the neighborhood of 0.8. When, however, as sometimes happens, the quotient at this time of day approaches 0.9, it must be assumed that sugar is being burned in the body, presumably from carbohydrate still circulating from the previous day's intake.

As can easily be seen, any special drain upon either fat or carbohydrate in the processes of the body will be indicated at once by a corresponding change in the respiratory quotient. This we shall have occasion to notice later on, in considering the source of the energy of muscle contraction. Further, the respiratory quotient will naturally change in harmony with transformations in the body which involve alterations in oxygen-content, without the oxygen of the inspired air being necessarily involved; as in the formation of a substance poor in oxygen, such as fat, from a substance rich in oxygen, such as carbohydrate. Moreover, the reversal of this reaction, as in the formation of sugar from proteid with a taking on of oxygen, will produce a corresponding effect upon the respiratory quotient. As Magnus-Levy has clearly pointed out, in the formation of fat from carbohydrate, carbon dioxide is produced in large amount without the oxygen of the inspired air being involved at all. In such a change, 100 grams of starch will yield about 42 grams. of fat, while at the same time 45 grams of carbon dioxide will be produced. This might cause the respiratory quotient to rise as high as 1.38. Again, in the formation of sugar from proteid, the respiratory quotient may sink very decidedly, the changes involved being accompanied by a taking on of oxygen from the air, without, however, any corresponding increase of carbon dioxide in the expired air. Assuming a manufacture of 60 grams of dextrose from 100 grams of proteid, i. e., from the non-nitrogenous moiety of the proteid molecule, a respiratory quotient of 0.613 would be possible. Thus, a diabetic patient, living upon a carbohydrate-free diet, consuming only proteid and fat, may show a respiratory quotient of 0.613-0.707. These illustrations will suffice to show how chemical alterations taking place in the body, involving transformations of proteid, fat, and carbohydrate of the tissues and of the food, may produce alterations in the respiratory quotient without necessarily being directly connected with intake of oxygen or output of carbon dioxide through the lungs; and how, conversely, the respiratory quotient becomes a factor of great significance in throwing light upon the character of the nutritive changes taking place in the body.

Among the various conditions that influence the energy exchange of the body, muscle work stands out as the most conspicuous. It needs no argument to convince one that all forms of muscular activity involve liberation of the energy stored up in the tissues of the body; and consequently that all work accomplished means chemical decomposition, in which complex molecules are broken down into simple ones with liberation of the contained energy, the energy exchange being proportional to the amount of work done. As we have soon, the basal energy exchange of the normal individual is ascertained by studying bis heat production while at rest best during sleep - without food, when involuntary muscle activity and heat production are at their lowest. The maximum energy exchange is seen in the individual at hard muscular work. Heat production is then at its highest, as can be ascertained by direct calorimetric observation; or, by studying the output of excretory products, which measure the extent of the oxidative processes from which comes the energy for the accomplishment of the work. As an illustration of the general effect of muscular work on the energy exchange of the body, we may cite a summary of some results reported by Atwater and Benedict,1 the figures given being average results, from several individuals, and covering different periods of time. Though not strictly comparable in all details, they are sufficiently so to illustrate the main principle.

1 Atwater and Benedict: Eiperimenti on the MeUboliira of Matter and Energy in the Human Body 1900-1902. Bulletin No. 136. Office of Expert. ment Stations, U. S. Department of Agriculture, 1903, p. 141.

Heat Given Off By Body, Including For Work Experiments The Heat Equivalent Of The External Muscular Work